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Nationwide Children's Transgenic & Embryonic Stem Cell

The Transgenic and Embryonic Stem Cell Core investigators with the production, maintenance and storage of genetically engineered mice.

The Core uses techniques including pronuclear microinjection of DNA, ES cell gene targeting, blastocyst injection of targeted ES cells, mouse strain rederivation by embryo transfer, de novo ES cell line generation from embryos and cryopreservation of both embryos and sperm.

The Core can also assist with more specialized techniques and train investigators to do their own experiments. The core has achieved a germline transmission in 100% of clones targeted within the Core ES cell facility.

Our resources include several lines of mice used for manipulating the mouse genome and/or tracking cell lineages as well as protocols and reagents for mouse model generation. The core is staffed by three trained research associates and currently the time between project request to commencement is typically less than 2-3 weeks.

Order a Transgenic and Embryonic Stem Cell Core Service

Contact the Stem Cell Core

Transgenic and Embryonic Stem Cell Core(614) 355-3483

To learn more about how to use the Transgenic and Embryonic Stem Cell Core, click here.

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