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Linan Wang, PhD Candidate

Award Name TL1

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Application of SILAC and LC-MS/MS to Determine the Global Proteomic Effect and Cross Talk of Histone Modifications in Yeast

Linan Wang, PhD CandidateThe Ohio State University Center for Clinical and Translational Science has awarded Linan Wang, PhD Candidate, with a TL1 Training Award for his research, “Application of SILAC and LC-MS/MS to Determine the Global Proteomic Effect and Cross Talk of Histone Modifications in Yeast.”

“Essentially, we are measuring what the post-translational modification of histones and their effects on the proteomic scale for the yeast organism.” Wang said.

Histones are highly alkaline proteins found in cell nuclei that package and order the DNA into structural units. This is wrapped around the DNA. Without histones, DNA would be much longer.

Wang is measuring what happens when a histone is modified and how that affects all the proteins in the cell using mass spectrometry. Wang then compares both the normal cell and the mutated cell and how the histone modification has changed the cell. Wang continues to scan through the mutant cell and analyzes the cell for medically significant data.

“It’s almost like flipping through the pages of a book, until you find something interesting,” said Wang. “You stop and look at it, then you move on until you find something else.”

Wang hopes to map out the modification sites of the histone in the cell and determine the function of each. “Hopefully my research can help search for drug targets, help find a diagnosis technique or predict an outcome of a disease such as cancer,” said Wang. “I wanted my research to be clinically significant, deal with new technology -- hence the mass spectrometry, and have a broader scope.”

Wang has presented his research at OSU Medical Center’s Research Day, the Ohio Mass Spectrometry Symposium conference and the American Society of Mass Spectrometry conferences. He plans to pass the project on to other clinicians in hopes of birthing other research.

By Charaun Little, Friday, January 27, 2012

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